Journal: REPRODUCTION
Article Title: Preeclampsia-related increase of interleukin-11 expression in human decidual cells
doi: 10.1530/rep-10-0263
Figure Lengend Snippet: Figure 1 Immunohistochemical analysis of IL11 expression in control and preeclamptic decidua. Representative micrographs of serial sections from control (A–C) and preeclamptic (D–F) specimens are shown. Vimentin immunostaining (red) identifies decidual cells in control (A) and preeclamptic (D) tissues. Cytokeratin immunostaining (red) identifies trophoblastic cells in control (C) and preeclamptic (F) tissues. IL11 immunostaining (brown) in decidual cells (arrows) was cytoplasmic and more intense than in interstitial trophoblast (arrowheads), where IL11 localization was mostly perimembranous (E inset). Between groups, IL11 immunostaining was greater in preeclamptic (E) versus control (B) decidual cells, while there was no significant difference among interstitial trophoblast. IL11 intensity HSCOREs in control and preeclamptic specimens (meanGS.E.M.) are shown (K); *, versus control decidual cells; †, versus group-respective decidual cells; P!0.05. Additionally, no statistical significance for IL11 HSCOREs was observed between chorionic villi of preeclamptic and control tissues (G and H). On the other hand, no significant difference was found for the IL11R expression between preeclamptic (J) versus control interstitial cytotrophoblasts (I). Parallel staining with a mouse isotype was used as a negative control for IL11 MAB (C and G; inset). Note that IL11 immunoreactivity in interstitial trophoblast was mostly perimembranous, especially in preeclampsia specimens (E, inset). The scale bar in panel F represents 50 mm for all panels, except the panel E inset, where it represents 20 mm.
Article Snippet: Excess serum was then removed, and serial sections were incubated with either mouse monoclonal IL11 antibody (R&D Systems, Minneapolis, MN, USA) at 10 mg/ml in 1% blocking horse serum in TBS, or monoclonal IL11R antibody (R&D Systems) at 10 mg/ml in 1% blocking horse serum in TBS, or mouse monoclonal anti-vimentin antibody (DakoCytomation, Carpinteria, CA, USA) at 1:100 dilution in TBS, or anti-cytokeratin antibody (DakoCytomation) at 1:100 dilution in TBS overnight in a humidified chamber at C4 8C.
Techniques: Immunohistochemical staining, Expressing, Control, Immunostaining, Staining, Negative Control
R&D Systems is a verified supplier 
R&D Systems manufactures this product 
